PXD018209 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Unbiased proteomic profiling uncovers a targetable GNAS/PKA/PP2A axis in SCLC |
| Description | Using unbiased kinase profiling, we identified protein kinase A (PKA) as an active kinase in small cell lung cancer (SCLC). Inhibition of PKA activity genetically, or pharmacologically by activation of the PP2A phosphatase, inhibits SCLC expansion in culture and in vivo. Conversely, GNAS (G-protein α subunit), a PKA activator that is genetically activated in a small subset of human SCLC, promotes SCLC development. Phosphoproteomic analyses identified many new PKA substrates and mechanisms of action. In particular, PKA activity is required for the propagation of SCLC cancer stem cells in transplantation studies. Broad proteomic analysis of recalcitrant cancers has the potential to uncover novel targetable signaling networks, such as the GNAS/PKA/PP2A axis in SCLC. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:07:26.675.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | John Gordan |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2020-03-26 00:22:40 | ID requested | |
| 1 | 2020-06-16 23:30:12 | announced | |
| ⏵ 2 | 2024-10-22 05:07:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: inhibitor beads, analog specific, kinase proteomics,small cell lung cancer |
Contact List
| John Gordan |
|---|
| contact affiliation | Medicine, University of California San Francisco |
| contact email | John.Gordan@ucsf.edu |
| lab head | |
| John Gordan |
|---|
| contact affiliation | University of California, San Francisco |
| contact email | john.gordan@ucsf.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018209 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018209
- Label: PRIDE project
- Name: Unbiased proteomic profiling uncovers a targetable GNAS/PKA/PP2A axis in SCLC
