PXD018154-2

PXD018154 is an original dataset announced via ProteomeXchange.

Title	20S proteasomes exported by malaria parasite promote its growth
Description	Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. While growing inside RBCs, Pf are known to secrete multipurpose extracellular vesicles (EVs), yet their influence on this parasite’s essential host cell, the RBC, remains unknown. Here we demonstrate that Pf parasites export within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve host RBCs by remodeling the cytoskeleton network. Furthermore, we identified four novel degradation targets of the exported 20S proteasome, the phosphorylated cytoskelatal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome export mechanism of Pf-iRBCs, which prime naïve RBC by altering membrane stiffness, to facilitate malaria parasite growth.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-04-06_02:18:38.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-23 15:27:13	ID requested
1	2021-01-03 23:42:20	announced
⏵ 2	2021-04-06 02:18:39	announced	2021-04-06: Updated publication reference for PubMed record(s): 33608523.
3	2024-10-22 05:17:12	announced	2024-10-22: Updated project metadata.

Dekel E, Yaffe D, Rosenhek-Goldian I, Ben-Nissan G, Ofir-Birin Y, Morandi MI, Ziv T, Sisquella X, Pimentel MA, Nebl T, Kapp E, Ohana Daniel Y, Karam PA, Alfandari D, Rotkopf R, Malihi S, Temin TB, Mullick D, Revach OY, Rudik A, Gov NS, Azuri I, Porat Z, Bergamaschi G, Sorkin R, Wuite GJL, Avinoam O, Carvalho TG, Cohen SR, Sharon M, Regev-Rudzki N, 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun, 12(1):1172(2021) [pubmed]

submitter keyword: Proteasome, malaria, phosphorylation

Neta Regev-Rudzki
contact affiliation	Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel
contact email	neta.regev-rudzki@weizmann.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD018154

PRIDE project URI

• PRIDE
  1. PXD018154
     1. Label: PRIDE project
     2. Name: 20S proteasomes exported by malaria parasite promote its growth