PXD018151 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deciphering and predicting CD4+ T cell immunodominance of Influenza virus hemagglutinin |
Description | The importance of CD4+ T helper (Th) cells is well appreciated in view of their essential role in the elicitation of antibody and cytotoxic T cell responses. However, the mechanisms that determine the selection of immunodominant epitopes within complex protein antigens remain elusive. Here, we used ex vivo stimulation of memory T cells and screening of naïve and memory T cell libraries, combined with T cell cloning and TCR sequencing, to dissect the human naïve and memory CD4+ T cell repertoire against the influenza pandemic H1 hemagglutinin (H1-HA). We found that naïve CD4+ T cells have a broad repertoire, being able to recognize naturally processed as well as cryptic peptides spanning the whole H1-HA sequence. In contrast, memory Th cells were primarily directed against just a few immunodominant peptides that were readily detected by mass spectrometry-based MHC-II peptidomics and predicted by structural accessibility analysis. Collectively, these findings reveal the presence of a broad repertoire of naïve T cells specific for cryptic H1-HA peptides, and demonstrate that antigen processing represents a major constraint determining immunodominance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-07 |
AnnouncementXML | Submission_2024-02-07_03:31:29.965.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Antonino Cassotta |
SpeciesList | scientific name: Influenza A virus (A/California/07-00002/2009(H1N1)); NCBI TaxID: 2060328; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-23 06:31:47 | ID requested | |
1 | 2020-06-28 23:15:09 | announced | |
⏵ 2 | 2024-02-07 03:31:30 | announced | 2024-02-07: Updated project metadata. |
Publication List
10.1084/jem.20200206; |
Cassotta A, Paparoditis P, Geiger R, Mettu RR, Landry SJ, Donati A, Benevento M, Foglierini M, Lewis DJM, Lanzavecchia A, Sallusto F, Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin. J Exp Med, 217(10):(2020) [pubmed] |
Keyword List
submitter keyword: peptidomics, Influenza, MHC-II, moDC, EBV-B, antigen presentation,human, hemagglutinin |
Contact List
Federica Sallusto |
contact affiliation | Institute for Research in Biomedicine Università della Svizzera italiana (USI), Faculty of Biomedical Sciences Via Vincenzo Vela 6 6500 Bellinzona, Switzerland |
contact email | federica.sallusto@irb.usi.ch |
lab head | |
Antonino Cassotta |
contact affiliation | Institute for Research in Biomedicine, Università della Svizzera italiana |
contact email | antonino.cassotta@irb.usi.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018151
- Label: PRIDE project
- Name: Deciphering and predicting CD4+ T cell immunodominance of Influenza virus hemagglutinin