PXD018129 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL |
Description | Chroococcidiopsis thermalis is an extremophilic cyanobacterium that is adaptable to a widerange of environments including deserts, where it can maintain viability after desiccation. This species is also capable of photoautotrophic growth under far-red light (FRL, 750 nm) The proteomic comparison in this study, in conjunction with topographical imaging of thylakoid membranes by atomic force microscopy, supports earlier findings that FRL growth is accompanied by the synthesis of alternative photosynthesis-related proteins. Under white light (WL), a number of phycobilisome (PBS) and photosystem (PS) subunits were predominant at levels 2 - 14-fold greater compared to FRL growth. Two antenna family proteins were markedly more abundant under WL, with a fold-difference of approximately 80. While WL-associated proteins persisted at relatively low abundance under FRL, an exclusive set of PBS and PS subunit isoforms was expressed under FRL. These proteins were undetectable under WL, in many cases encoded in a discrete FRL-associated gene cluster. In line with the expected higher energy capture rate under WL, complexes associated with electron transport (eg. cytochrome b6f) and the ATP synthase were 1.2 – 3.4 times more abundant than under FRL. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-01 |
AnnouncementXML | Submission_2022-03-01_08:31:42.271.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Philip Jackson |
SpeciesList | scientific name: Chroococcidiopsis thermalis PCC 7203; NCBI TaxID: 251229; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-20 14:08:10 | ID requested | |
⏵ 1 | 2022-03-01 08:31:43 | announced | |
Publication List
MacGregor-Chatwin C, N, ü, rnberg DJ, Jackson PJ, Vasilev C, Hitchcock A, Ho MY, Shen G, Gisriel CJ, Wood WHJ, Mahbub M, Selinger VM, Johnson MP, Dickman MJ, Rutherford AW, Bryant DA, Hunter CN, Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation. Sci Adv, 8(6):eabj4437(2022) [pubmed] |
Keyword List
submitter keyword: Chroococcidiopsis thermalis, thylkoid, photosystem, phycobilisome |
Contact List
ChristopherNeil Hunter |
contact affiliation | Molecular Biology and Biotechnology, University of Sheffield |
contact email | c.n.hunter@sheffield.ac.uk |
lab head | |
Philip Jackson |
contact affiliation | Department of Chemical and Biological Engineering, University of Sheffield |
contact email | p.j.jackson@sheffield.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018129
- Label: PRIDE project
- Name: NanoLC-MS/MS DDA Chroococcidiopsis thermalis PCC 7203 thylkoids WL vs. FRL