PXD018123-1

PXD018123 is an original dataset announced via ProteomeXchange.

Title	E.coli Ribosome protein composition
Description	Ribosomes are essential macromolecular complexes conducting protein biosynthesis in all domains of life. Cells can have heterogeneous ribosomes, i.e. ribosomes with various ribosomal RNA (rRNA) and ribosomal protein (r-protein) composition. However, the functional importance of heterogeneous ribosomes has remained elusive. One of the possible sources for ribosome heterogeneity is provided by paralogous r-proteins. In E. coli, ribosomal protein bL31 has two paralogs: bL31A encoded by rpmE and bL31B encoded by ykgM. This study investigates phenotypic effects of these ribosomal protein paralogs using bacterial strains expressing only bL31A or bL31B. We show that bL31A confers higher fitness to E. coli under lower temperatures. In addition, bL31A and bL31B have different effects on translation reading frame maintenance and apparent translation processivity in vivo as demonstrated by dual luciferase assay. In general, this study demonstrates that ribosomal protein paralog composition (bL31A versus bL31B) can affect cell growth and translation outcome.
HostingRepository	PRIDE
AnnounceDate	2020-10-20
AnnouncementXML	Submission_2020-10-20_01:05:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018123
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kaspar Reier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Label:2H(4); Oxidation; Label:13C(6); monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-20 07:00:09	ID requested
⏵ 1	2020-10-20 01:05:33	announced

Lilleorg S, Reier K, Vol, õ, nkin P, Remme J, Liiv A, Phenotypic effects of paralogous ribosomal proteins bL31A and bL31B in E. coli. Sci Rep, 10(1):11682(2020) [pubmed]

submitter keyword: Escherichia coli, Ribosome, LC-MS/MS

Tiina Tamm
contact affiliation	Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia
contact email	ttamm@ut.ee
lab head
Kaspar Reier
contact affiliation	University of Tatu
contact email	kaspar.reier@ut.ee
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018123
     1. Label: PRIDE project
     2. Name: E.coli Ribosome protein composition