PXD018113 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Antibody RING-Mediated Destruction (ARMeD) of endogenous proteins |
Description | To understand the cellular function of a gene the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the half-life of the protein. Therefore, complete gene deletions and RNA interference of proteins with a very long half-life risk obscuring the direct consequences via compensatory or secondary cellular responses. We therefore sought to develop a single-component system that could induce the rapid degradation of the specific endogenous protein itself. A genetically introduced inducible construct of the RING domain of the ubiquitin E3 ligase RNF4 with a protein-specific camelid nanobody mediates destruction of the target by the ubiquitin proteasome system, a process we describe as Antibody RING-Mediated Destruction (ARMeD). The technique is acutely specific as we observed no off-target protein destruction. Furthermore, bacterially produced nanobody-RING fusion proteins electroporated into cells induce degradation of target within minutes. With the increasing availability of protein-specific nanobodies this method will allow the rapid and specific degradation of a wide range of endogenous proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-29 |
AnnouncementXML | Submission_2020-05-29_00:31:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mike Tatham |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-19 16:40:52 | ID requested | |
⏵ 1 | 2020-05-29 00:31:51 | announced | |
2 | 2024-10-22 05:06:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Ibrahim AFM, Shen L, Tatham MH, Dickerson D, Prescott AR, Abidi N, Xirodimas DP, Hay RT, Antibody RING-Mediated Destruction of Endogenous Proteins. Mol Cell, 79(1):155-166.e9(2020) [pubmed] |
Keyword List
submitter keyword: RING Ubiquitin Nanobody Endogenous Degradation |
Contact List
Ronald T. Hay |
contact affiliation | University of Dundee |
contact email | r.t.hay@dundee.ac.uk |
lab head | |
Mike Tatham |
contact affiliation | University of Dundee |
contact email | m.tatham@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018113
- Label: PRIDE project
- Name: Antibody RING-Mediated Destruction (ARMeD) of endogenous proteins