PXD018105-1
PXD018105 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | SILAC-based quantitative MS analysis of proteome labeled by covalent hDM2 inhibitor |
Description | SJSA1 cells grown in isotopically "light" or "heavy" medium were treated with Nutlin-3 (10 µM, 4 h). After removal of Nutlin-3 by washing, "light" amino acid-labeled cells were treated with DMSO or covalent hDM2 inhibitor (1 µM) in the presence of Nutlin-3 (10 µM) for 1 h, whereas "heavy" amino acid-labeled cells were treated with covalent hDM2 inhibitor (1 µM). Each cell population were lysed and combined in a 1:1 ratio. The covalent hDM2 inhibitor-modified proteins were conjugated to azide-PEG3-desthiobiotin and purified with Neutravidin beads. Enriched proteins were then subjected to in-gel trypsinization and the resultant peptides were analyzed by LC-MS/MS. |
HostingRepository | jPOST |
AnnounceDate | 2021-03-19 |
AnnouncementXML | Submission_2021-03-18_08:00:04.826.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tsuyoshi Ueda |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-03-19 04:45:06 | ID requested | |
⏵ 1 | 2021-03-18 08:00:05 | announced | |
2 | 2022-09-18 03:03:39 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: human, SILAC |
Contact List
Itaru Hamachi | |
---|---|
lab head | |
Tsuyoshi Ueda | |
contact affiliation | Kyoto University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000767 |