PXD018060-1

PXD018060 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Rickettsia akari: the proteome revealed a 44kDa-OMP as novel biomarker for Rickettsialpox
Description	We identified 296 unique proteins separated by shotgun proteomics technique, 30 surface exposed proteins using biotinylation and 67 proteins by Triton X-114 detergent phase partitioning. Combination of different separation techniques with divers in silico analyses revealed 25 possibly beta-barrel outer membrane proteins and 11 proteins enclosing signal peptide sequence. Among these signal peptide containing surface proteins we detected several proteins affecting virulence, like outer membrane protein B, 60 kDa chaperonin GroEL, putative surface antigen and peptidoglycan-associated lipoprotein. The most interesting finding is a unique antigenic 44kDa uncharacterized protein, reacting with serum specimens from R. akari infected patients. This protein is characterized for the first time in present study.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_03:15:46.466.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018060
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fernando Zuniga
SpeciesList	scientific name: Rickettsia akari; NCBI TaxID: 786;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q-Tof Ultima

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-16 14:13:33	ID requested
⏵ 1	2024-10-11 03:15:47	announced

Publication List

10.6019/PXD018060;
10.1186/s12866-020-01877-6;
Csicsay F, Flores-Ramirez G, Zu, ñ, iga-Navarrete F, Barto, š, ov, á M, Fu, č, í, kov, á A, Pajer P, Dresler J, Š, kult, é, ty Ľ, Quevedo-Diaz M, kDa-OMP as a potential biomarker for Rickettsialpox diagnosis. BMC Microbiol, 20(1):200(2020) [pubmed]

10.6019/PXD018060;

10.1186/s12866-020-01877-6;

Csicsay F, Flores-Ramirez G, Zu, ñ, iga-Navarrete F, Barto, š, ov, á M, Fu, č, í, kov, á A, Pajer P, Dresler J, Š, kult, é, ty Ľ, Quevedo-Diaz M, kDa-OMP as a potential biomarker for Rickettsialpox diagnosis. BMC Microbiol, 20(1):200(2020) [pubmed]

Keyword List

submitter keyword: Rickettsia akari, outer membrane proteins, Liquid Chromatography Mass Spectrometry

submitter keyword: Rickettsia akari, outer membrane proteins, Liquid Chromatography Mass Spectrometry

Contact List

Ludovit Skultety
contact affiliation	Head of proteomic facility, Institute of Virology, BMC, Slovak Academy of Sciences, Bratislava, Slovak Republic
contact email	Ludovit.Skultety@savba.sk
lab head
Fernando Zuniga
contact affiliation	BMC SAV
contact email	feznarr@icloud.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018060
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018060

PRIDE project URI

Repository Record List

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