PXD018051-1

PXD018051 is an original dataset announced via ProteomeXchange.

Title	Comparing Biological and Pathological Factors Affecting Osteocalcin Concentrations in Archaeological Skeletal Remains
Description	Paleoproteomics and the study of ancient proteins has become an important consideration in bioarchaeological research as we seek to understand the relationship between the physical skeleton and its underlying biochemistry. Osteocalcin (OC) is an abundant, non-collagenous protein that is accessible archaeologically due to its affinity for hydroxyapatite in bone. Manufactured by the osteoblast cells during normal bone metabolism, osteocalcin is inherently linked to the process of bone remodelling. Clinical studies have consistently shown the impact of biological (i.e., sex and age) and pathological (i.e., disease) factors on osteocalcin concentrations within the body; however, this connection has yet to be fully explored bioarchaeologically. For this study, 46 individuals from the Danish Sct. Albert’s cemetery (AD 1250-1536) were analyzed with cortical bone samples harvested from the femur. Each sample was demineralized and quantified using established paleoproteomic methods (ELISA) and tested for diagenesis using FTIR-ATR. 4 samples were validated by LC-MS. No significant differences were found in the osteocalcin concentrations between adult males and females. However, males had a wider range of osteocalcin variability perhaps explained in part by their increased prevalence of pathological conditions characterized by bone remodelling. Similarly, non-adults had a wide range of osteocalcin variability but there were no significant differences when compared to the adult mean. No correlations were found between osteocalcin concentrations and diagenesis suggesting that the protein can survive across a range of temporal periods and variable burial environments. While no clear pattern emerged between osteocalcin concentrations and biological and/or pathological factors, this study is significant in that it further explores the use of paleoproteomics in bioarchaeological contexts and how we may unite our macroscopic and biochemical assessments of human skeletal remains.
HostingRepository	PRIDE
AnnounceDate	2020-10-13
AnnouncementXML	Submission_2020-10-13_01:05:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meaghan Mackie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 05:12:10	ID requested
⏵ 1	2020-10-13 01:05:20	announced
2	2024-10-22 05:13:21	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: human, bone, osteocalcin, archaeology, palaeoproteomics, LC-MS

Matthew Collins
contact affiliation	Section for Evolutionary Genomics, GLOBE Institute, University of Copenhagen, Copenhagen, Denmark; McDonald Institute for Archaeological Research, University of Cambridge, Cambridge, UK.
contact email	matthew.collins@palaeome.org
lab head
Meaghan Mackie
contact affiliation	University of Copenhagen
contact email	meaghan@palaeome.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018051
     1. Label: PRIDE project
     2. Name: Comparing Biological and Pathological Factors Affecting Osteocalcin Concentrations in Archaeological Skeletal Remains