PXD018049 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Feedback regulation in EGFR signaling involves IRF2BP proteins and temporal control by (de)SUMOylation |
Description | Molecular switches are essential modules in signaling networks by enabling a quick response to environmental changes. Here, we describe a role for small ubiquitin-like-modifier SUMO as a molecular switch in epidermal growth factor receptor (EGFR) signaling. Using quantitative mass spectrometry, we compared the endogenous SUMO-proteomes of Hela cells before and after EGF-stimulation. Thereby, we identified a small group of transcriptional repressors, including IRF2BP1, IRF2BP2 and IRF2BPL as novel players in EGFR signaling, which lost SUMO transiently within 15 min of EGF treatment. We found 38 genes whose expression within the first hour of EGF treatment was altered in cells expressing SUMOylation deficient IRF2BP1, including Dual specific phosphatase 1 (DUSP1), an important feedback regulator of EGFR signalling. We show that IRF2BP1 binds to the proximal promoter of DUSP1, whose activation requires IRF2BP1 deSUMOylation. Thus, the SUMO-dependent molecular switch of IRF2BP1 activity is important to finely tune EGFR signaling |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-14 |
AnnouncementXML | Submission_2021-04-13_22:47:29.074.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uwe PLessmann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-13 05:10:50 | ID requested | |
1 | 2021-04-13 05:12:59 | announced | |
⏵ 2 | 2021-04-13 22:47:29 | announced | 2021-04-14: Updated project metadata. |
Publication List
Barysch SV, Stankovic-Valentin N, Miedema T, Karaca S, Doppel J, Nait Achour T, Vasudeva A, Wolf L, Sticht C, Urlaub H, Melchior F, Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling. EMBO Rep, 22(3):e49651(2021) [pubmed] |
10.15252/EMBR.201949651; |
Keyword List
submitter keyword: IRF2BP1, DUSP1, transcription, IRF2BP2,EGF, feedback inhibitor, EGFR, SUMO, regulation |
Contact List
Henning Urlaub |
contact affiliation | Bioanalytical Mass Spectromerty Group Max Planck Institute for Biophysical Chemnistry Goettingen, Germany |
contact email | hurlaub@gwdg.de |
lab head | |
Uwe PLessmann |
contact affiliation | Max Planck Intitute for biophysical Chemnistry |
contact email | uplessm@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018049
- Label: PRIDE project
- Name: Feedback regulation in EGFR signaling involves IRF2BP proteins and temporal control by (de)SUMOylation