PXD018048-2

PXD018048 is an original dataset announced via ProteomeXchange.

Title	HepG2 GalNAc-T1/2 Bump-and-hole secretome glycopeptide data
Description	Engineered GalNAc-T glycosyltransferases were used to incorporate a chemically modified GalNAC analog into the secretome of HepG2 cells. The chemical modification included a bioorthogonal alkyne tag that allowed for introduction of a clickable, acid-cleavable biotin-picolyl-azide. Glycoproteins were enriched using Streptavidin, and on-bead digestion yielded solid phase-bound glycopeptides that were released with acid. Glycopeptides were analysed.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-04-26_23:59:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Schumann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; complex glycosylation; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-13 05:10:44	ID requested
1	2020-04-21 23:21:34	announced
⏵ 2	2020-04-26 23:59:19	announced	2020-04-27: Updated publication reference for PubMed record(s): 32325029.

Schumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR, Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Mol Cell, 78(5):824-834.e15(2020) [pubmed]

ProteomeXchange project tag: Human Proteome Project

submitter keyword: Human, HepG2, liver, secretome, glycopeptide, bioorthogonal, click, GalNAc-T

Benjamin Schumann
contact affiliation	Department of Chemistry, Imperial College London, UK; Chemical Glycobiology Laboratory, The Francis Crick Institute, London
contact email	b.schumann@imperial.ac.uk
lab head
Benjamin Schumann
contact affiliation	Imperial College London, The Francis Crick Institute
contact email	b.schumann@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD018048

PRIDE project URI

• PRIDE
  1. PXD018048
     1. Label: PRIDE project
     2. Name: HepG2 GalNAc-T1/2 Bump-and-hole secretome glycopeptide data