PXD018025 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Testing serum specificity for bacterial antigens in a mouse model of spontaneous colitis |
Description | Identification of bacterial antigens within cecal bacterial lysate was performed by using serum antibodies from control and DC-LMP1/CD40 animals for immunoprecipitation followed by label-free liquid chromatography tandem mass spectrometry (LC-MS/MS). Serum antibodies were coupled to beads and incubated with cecal bacterial lysate to bind target proteins. Upon immunoprecipitation, on-beads digestion of proteins followed by LC-MS/MS was performed. The resulting peak intensities were used for intensity-based quantification (iBAQ). Proteins identified with a fold change > 2 and a p-value < 0.05 were considered for further analyses. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-25 |
AnnouncementXML | Submission_2021-02-25_02:43:30.199.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignasi Forne |
SpeciesList | scientific name: Helicobacter hepaticus; NCBI TaxID: 32025; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-12 04:43:02 | ID requested | |
1 | 2021-02-25 01:33:45 | announced | |
⏵ 2 | 2021-02-25 02:43:31 | announced | 2021-02-25: Updated project metadata. |
Publication List
Friedrich V, Forn, é I, Matzek D, Ring D, Popper B, Jochum L, Spriewald S, Straub T, Imhof A, Krug A, Stecher B, Brocker T, is required for immune targeting of bacterial heat shock protein 60 and fatal colitis in mice. Gut Microbes, 13(1):1-20(2021) [pubmed] |
10.1080/19490976.2021.1882928; |
Keyword List
submitter keyword: spontaneous colitis, Helicobacter hepaticus |
Contact List
Prof. Thomas Brocker |
contact affiliation | Institute for Immunology, BioMedical Center, Faculty of Medicine, LMU Munich, 82152 Munich, Germany |
contact email | tbrocker@med.uni-muenchen.de |
lab head | |
Ignasi Forne |
contact affiliation | Biomedical Center-LMU |
contact email | ignasi.forne@lrz.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD018025 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018025
- Label: PRIDE project
- Name: Testing serum specificity for bacterial antigens in a mouse model of spontaneous colitis