PXD018022-1

PXD018022 is an original dataset announced via ProteomeXchange.

Title	Quantitative proximity proteomics suggests a phytotransferrin-mediated cell surface-to-chloroplast iron trafficking axis in marine diatoms
Description	Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is internalized via endocytosis, however proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model diatom Phaeodactylum tricornutum. Proteins encoded by poorly characterized iron-sensitive genes were identified including three that are expressed from a chromosomal gene cluster. Two of them showed unambiguous colocalization with phytotransferrin adjacent to the chloroplast. Further phylogenetic, domain, and biochemical analyses suggest their involvement in intracellular iron processing. Proximity proteomics holds enormous potential to glean new insights into iron acquisition pathways and beyond in these evolutionarily, ecologically, and biotechnologically important microalgae.
HostingRepository	PRIDE
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-02_15:43:04.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jernej Turnsek
SpeciesList	scientific name: Phaeodactylum tricornutum CCAP 1055/1; NCBI TaxID: 556484;
ModificationList	biotinylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 04:04:09	ID requested
⏵ 1	2021-03-02 15:43:04	announced
2	2024-10-22 05:19:36	announced	2024-10-22: Updated project metadata.

Turn, š, ek J, Brunson JK, Viedma MDPM, Deerinck TJ, Hor, á, k A, Oborn, í, k M, Bielinski VA, Allen AE, Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway. Elife, 10():(2021) [pubmed]

submitter keyword: Ocean, Iron cycle, Diatoms, Phaeodactylum tricornutum, Phytotransferrin, Endocytosis, Metal trafficking, Chloroplast, Proximity proteomics, APEX2

Andrew Ellis Allen
contact affiliation	1. Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, CA 92037, USA 2. Integrative Oceanography Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA
contact email	aallen@jcvi.org
lab head
Jernej Turnsek
contact affiliation	Harvard University
contact email	turnsek@fas.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018022
     1. Label: PRIDE project
     2. Name: Quantitative proximity proteomics suggests a phytotransferrin-mediated cell surface-to-chloroplast iron trafficking axis in marine diatoms