PXD018016-2

PXD018016 is an original dataset announced via ProteomeXchange.

Title	The RHOA-specific GTPase activating protein ARHGAP45 controls chemokine-driven migration of naïve T and B cells and of hematopoietic progenitors across blood endothelium
Description	T cell recirculation and organ entry involve diapedesis through cytoskeleton remodeling. Part of this process is controlled by guanosine exchange factors (GEFs) and GTPase activating proteins (GAPs) that promote active and inactive forms of Rho family of GTPases. Among GAPs, we identified the ARHGAP45, also known as HMHA1 (human minor histocompatibility antigen 1) and determined its interactome by affinity purification coupled to quantitative mass spectrometry (AP-MS) in Jurkat cells. This dataset contains results of AP-MS of Jurkat cells expressing One-Strep-tagged (OST) ARHGAP45 prior to and after TCR stimulation. Each AP-MS purification of OST- protein is associated with a corresponding control (WT Jurkat) at the same time point of stimulation. For each time point, three independent biological replicates were performed and each biological replicate was analyzed in duplicate by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:31.296.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 03:18:31	ID requested
1	2021-02-04 00:01:10	announced
⏵ 2	2024-10-22 05:18:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MS, ARHGAP45, interactome

Marseille Proteomics
contact affiliation	Centre d'Immunologie de Marseille, Aix-Marseille Univerité, Inserm, CNRS, Centre d'immunophénomique, Centre de Recherche en Cancérologie de Marseille, CRCM, Institut Paoli Calmettes, MARSEILLE PROTEOMIQUE
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018016
     1. Label: PRIDE project
     2. Name: The RHOA-specific GTPase activating protein ARHGAP45 controls chemokine-driven migration of naïve T and B cells and of hematopoietic progenitors across blood endothelium