PXD018015-1

PXD018015 is an original dataset announced via ProteomeXchange.

Title	A unique cell wall synthetic response evoked by glucosamine determines pathogenicity-associated fungal cellular differentiation
Description	The yeast-to-hypha transition is tightly associated with pathogenicity in many human pathogenic fungi, such as the model fungal pathogen Cryptococcus neoformans, which is responsible for approximately 180,000 deaths annually. In this pathogen, the yeast-to-hypha transition can be initiated by distinct stimuli: mating stimulation or glucosamine (GlcN), the monomer of cell wall chitosan. However, it remains poorly understood how the signal specificity for Cryptococcus morphological transition by disparate stimuli is ensured. Here, by integrating temporal expression signature analysis and phenome-based clustering evaluation, we demonstrate that GlcN specifically triggers a unique cellular response, which acts as a critical determinant underlying the activation of GlcN-induced filamentation (GIF). This cellular response is defined by an unusually hyperactive cell wall synthesis that is highly ATP-consuming. A novel cell surface protein Gis1 was identified as the indicator molecule for the GlcN-induced cell wall response. The Mpk1-directed cell wall pathway critically bridges global cell wall gene induction and intracellular ATP supply, ensuring the Gis1-dependent cell wall response and the stimulus specificity of GIF. We further reveal that the ability of Mpk1 to coordinate the cell wall response and GIF activation is conserved in different Cryptococcus pathogens. Phosphoproteomics-based profiling together with genetic and phenotypic analysis revealed that the Mpk1 kinase mediates the regulatory specificity of GIF through a coordinated downstream regulatory network centered on Skn7 and Crz1. Overall, our findings discover an unprecedented and conserved cell wall biosynthesis-dependent fungal differentiation commitment mechanism, which enables the signal specificity of pathogenicity-related dimorphism induced by GlcN in Cryptococcus pathogens.
HostingRepository	PRIDE
AnnounceDate	2021-09-15
AnnouncementXML	Submission_2021-09-15_02:33:34.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Ding
SpeciesList	scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-12 03:18:30	ID requested
⏵ 1	2021-09-15 02:33:35	announced

Dataset with its publication pending

submitter keyword: Cryptococcus neoformans, Glucosamine, yeast-to-hypha transition, cell wall

Linqi Wang
contact affiliation	State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
contact email	wanglq@im.ac.cn
lab head
Hao Ding
contact affiliation	Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
contact email	dinghaov587@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018015
     1. Label: PRIDE project
     2. Name: A unique cell wall synthetic response evoked by glucosamine determines pathogenicity-associated fungal cellular differentiation