PXD018007 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | tRNA binding to KEOPS human protein complex |
Description | N6-theronylcarbomyl adenosine (t6A) is a universal tRNA posttranscriptional modification that promotes translation fidelity and is therefore required for the fitness of virtually all living cells. Kae1, the t6A modifying enzyme in eukaryotes and archea, resides within the KEOPS complex with three auxiliary subunits of unknown function namely Cgi121, Bud32 and Pcc1. Through biochemical and X-ray crystallographic analyses we show that Cgi121 functions to recruit substrate tRNA to KEOPS via its universal 3’-CCA tail. Incorporation of the Cgi121-tRNA structure into a composite model of KEOPS reveals an extended tRNA contact surface that surprisingly spans all four subunits. Comprehensive mutational and functional analyses validate the relevance of the extended tRNA-binding surface in vivo and in vivo. Together this work provides insight into the t6A catalytic cycle and its regulation through the cooperative action of all four KEOPS subunit through direct contacts with tRNA. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-11 |
AnnouncementXML | Submission_2020-12-10_23:15:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dave Schriemer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-12 00:22:42 | ID requested | |
⏵ 1 | 2020-12-10 23:15:27 | announced | |
Publication List
Beenstock J, Ona SM, Porat J, Orlicky S, Wan LCK, Ceccarelli DF, Maisonneuve P, Szilard RK, Yin Z, Setiaputra D, Mao DYL, Khan M, Raval S, Schriemer DC, Bayfield MA, Durocher D, Sicheri F, A substrate binding model for the KEOPS tRNA modifying complex. Nat Commun, 11(1):6233(2020) [pubmed] |
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
submitter keyword: tRNA, KEOPS, HX-MS |
Contact List
David Christopher Schriemer |
contact affiliation | Dept. of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
Dave Schriemer |
contact affiliation | Dept. Biochemistry and Molecular Biology, University of Calgary |
contact email | dschriem@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018007
- Label: PRIDE project
- Name: tRNA binding to KEOPS human protein complex