PXD018001-2

PXD018001 is an original dataset announced via ProteomeXchange.

Title	Drosophila melanogaster eye Proteomics
Description	Absolute (molar) quantification determines proteins stoichiometry in complexes, networks and metabolic pathways. We employed MS Western workflow to determine molar abundances of proteins critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. Each protein was independently quantified with 2 to 4 proteotypic peptides with the coefficient of variation of less than 15 %, better than 1000-fold dynamic range and sub-femtomole sensitivity. We determined molar abundances and stoichiometric ratios of the components of the PT machinery and the rhabdomere, and how they are changing when rhabdomere morphogenesis is perturbed by genetic manipulation of the evolutionary conserved gene crumbs (crb).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:09:23.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bharath Kumar Raghuraman
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 09:43:13	ID requested
1	2020-07-18 18:40:46	announced
⏵ 2	2024-10-22 05:09:28	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201900049;

Raghuraman BK, Hebbar S, Kumar M, Moon H, Henry I, Knust E, Shevchenko A, Absolute Quantification of Proteins in the Eye of Drosophila melanogaster. Proteomics, 20(23):e1900049(2020) [pubmed]

submitter keyword: QConCAT, Drosophila

Andrej Shevchenko
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics
contact email	shevchenko@mpi-cbg.de
lab head
Bharath Kumar Raghuraman
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics
contact email	raghuram@mpi-cbg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018001

PRIDE project URI

• PRIDE
  1. PXD018001
     1. Label: PRIDE project
     2. Name: Drosophila melanogaster eye Proteomics