PXD017997-2

PXD017997 is an original dataset announced via ProteomeXchange.

Title	Multi-omics reveals the lipid inventory of synaptic junctions and altered lipid signaling in environmental enrichment
Description	Membrane lipids are essential components of synaptic junctions that have key functions in neurotransmission. In the first part of the study, we provide a quantitative lipid inventory of mouse and rat synaptic junctions. We next used a simultaneous multi-omics extraction and analysis workflow termed SIMPLEX to probe the interplay of proteins and lipids in synaptic signal transduction. We found that SIMPLEX is suitable to generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach revealed that in mice exposed to an enriched environment reduced endocannabinoid signaling is linked to increased surface expression of AMPAR in a subset of Cannabinoid-receptor 1 positive synapses, a mechanism which likely regulates synaptic strength in an input-specific manner.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:13:38.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Canan Has
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 06:47:15	ID requested
1	2023-11-13 21:39:02	announced
⏵ 2	2023-11-14 09:13:40	announced	2023-11-14: Updated project metadata.

Borgmeyer M, Coman C, Has C, Sch, ö, tt HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, Dumenieu M, Schweizer M, Chocholou, š, kov, á M, Hol, č, apek M, Mikhaylova M, Kreutz MR, Ahrends R, Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment. Cell Rep, 37(1):109797(2021) [pubmed]

submitter keyword: Enriched Environment,Lipidomics, Multi-omics, Endocannabinoid Signaling, Synaptic Junctions

Robert Ahrends
contact affiliation	University of Vienna Department of Analytical Chemistry
contact email	robert.ahrends@univie.ac.at
lab head
Canan Has
contact affiliation	Leibniz-Institut für Analytische Wissenschaften (ISAS)
contact email	cananhas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD017997

PRIDE project URI

• PRIDE
  1. PXD017997
     1. Label: PRIDE project
     2. Name: Multi-omics reveals the lipid inventory of synaptic junctions and altered lipid signaling in environmental enrichment