PXD017983-1

PXD017983 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation of PTEN coupled Nano-LC−ESI-MS/MS
Description	Nuclear localization of PTEN is essential for its tumor suppressive role, and loss of nuclear PTEN is more prominent than cytoplasmic PTEN in many kinds of cancers. However, nuclear PTEN-specific regulatory mechanisms were rarely reported. Based on the finding that nuclear PTEN is more unstable than cytoplasmic PTEN. Thus, 293T cells were transiently transfected with plasmid encoding Flag-tagged PTEN along with empty vector, followed by affinity purification using an anti-Flag antibody, and the bound proteins were analyzed by liquid chromatography with tandem mass spectrometry (LC−MS/MS). Here we identify that F-box only protein 22 (FBXO22) induces ubiquitylation of nuclear but not cytoplasmic PTEN at lysine 221, which is responsible for the degradation of nuclear PTEN.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-04-22_04:44:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017983
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ge mengkai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 00:35:22	ID requested
⏵ 1	2020-04-22 04:44:18	announced

Ge MK, Zhang N, Xia L, Zhang C, Dong SS, Li ZM, Ji Y, Zheng MH, Sun J, Chen GQ, Shen SM, FBXO22 degrades nuclear PTEN to promote tumorigenesis. Nat Commun, 11(1):1720(2020) [pubmed]

submitter keyword: 293T,PTEN,immunoprecipitation,Nano-LC−ESI-MS/MS

Chen Guo-Qiang
contact affiliation	Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Rui-Jin Hospital, and State Key Laboratory of Oncogenes and Related Genes and Chinese Academy of Medical Sciences Research Unit (NO.2019RU043), Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
contact email	chengq@shsmu.edu.cn
lab head
ge mengkai
contact affiliation	SJTU
contact email	gemengk@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017983

PRIDE project URI

• PRIDE
  1. PXD017983
     1. Label: PRIDE project
     2. Name: Immunoprecipitation of PTEN coupled Nano-LC−ESI-MS/MS