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PXD017966-1

PXD017966 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCarbon source dependent reprograming of anaerobic metabolism in Staphylococcus aureus
DescriptionTo be a successful pathogen, S. aureus has to adapt its metabolism to the typically oxygen- and glucose-limited environment of the host. Under fermenting conditions and in the presence of glucose S. aureus uses glycolysis in order to generate ATP via substrate level phosphorylation and mainly lactic acid fermentation to maintain redox balance by oxidizing NADH equivalents. However, it is unclear how S. aureus proceeds under anoxic conditions when also glucose is limited, likely representing the bona-fide situation in the host. Using a combination of proteome, transcription and metabolome analyses, we show that in the absence of glucose and oxygen, pyruvate as being a model metabolite for a non-glycolytic carbon source is channeled towards the production of acetyl-CoA (AcCoA) in a pyruvate formate-lyase (PflB)-dependent reaction to produce ATP and acetate. This process critically depends on inactivation of the catabolite control protein A (CcpA), leading to upregulation of pflB transcription. Thus, we describe a switch prioritizing acetate over lactate fermentation when glucose is absent. Under these conditions also ethanol production via the alcohol dehydrogenases AdhE and Adh1 is repressed in order to prevent wasteful consumption of AcCoA. Taken together, our study provides insights into the metabolic framework underlying the adaption of S. aureus to adverse host conditions.
HostingRepositoryPRIDE
AnnounceDate2021-02-15
AnnouncementXMLSubmission_2021-02-14_23:05:07.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniela Zuehlke
SpeciesList scientific name: Staphylococcus aureus subsp. aureus COL; NCBI TaxID: 93062;
ModificationListiodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-10 03:13:13ID requested
12021-02-14 23:05:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: S. aureus, fermentation, mass spectrometry
Contact List
Katharina Riedel
contact affiliationUniversisty of Greifswald, Institute of Microbiology, Department for Microbial Physiology and Molecular Biology
contact emailriedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliationErnst Moritz Arndt University Greifswald, Institute of Microbiology
contact emailzuehlke@uni-greifswald.de
dataset submitter
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Dataset FTP location
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