PXD017966 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Carbon source dependent reprograming of anaerobic metabolism in Staphylococcus aureus |
Description | To be a successful pathogen, S. aureus has to adapt its metabolism to the typically oxygen- and glucose-limited environment of the host. Under fermenting conditions and in the presence of glucose S. aureus uses glycolysis in order to generate ATP via substrate level phosphorylation and mainly lactic acid fermentation to maintain redox balance by oxidizing NADH equivalents. However, it is unclear how S. aureus proceeds under anoxic conditions when also glucose is limited, likely representing the bona-fide situation in the host. Using a combination of proteome, transcription and metabolome analyses, we show that in the absence of glucose and oxygen, pyruvate as being a model metabolite for a non-glycolytic carbon source is channeled towards the production of acetyl-CoA (AcCoA) in a pyruvate formate-lyase (PflB)-dependent reaction to produce ATP and acetate. This process critically depends on inactivation of the catabolite control protein A (CcpA), leading to upregulation of pflB transcription. Thus, we describe a switch prioritizing acetate over lactate fermentation when glucose is absent. Under these conditions also ethanol production via the alcohol dehydrogenases AdhE and Adh1 is repressed in order to prevent wasteful consumption of AcCoA. Taken together, our study provides insights into the metabolic framework underlying the adaption of S. aureus to adverse host conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-15 |
AnnouncementXML | Submission_2021-02-14_23:05:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Staphylococcus aureus subsp. aureus COL; NCBI TaxID: 93062; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-10 03:13:13 | ID requested | |
⏵ 1 | 2021-02-14 23:05:07 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: S. aureus, fermentation, mass spectrometry |
Contact List
Katharina Riedel |
contact affiliation | Universisty of Greifswald, Institute of Microbiology, Department for Microbial Physiology and Molecular Biology |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | Ernst Moritz Arndt University Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017966
- Label: PRIDE project
- Name: Carbon source dependent reprograming of anaerobic metabolism in Staphylococcus aureus