PXD017945-2

PXD017945 is an original dataset announced via ProteomeXchange.

Title	Data-dependent and independent acquisition of complex human-Salmonella mixed proteomes
Description	Viewing the scarce amount of protein material coming from the bacterial pathogen in infection models and despite the availability of contemporary, highly sensitive and fast scanning mass spectrometers, the power requirement still not suffices to study the host and pathogen proteomes simultaneously. In the present work we aimed to establish a DIA mass spectrometry workflow for improving the protein identification and quantification of LC-MS/MS, particularly in case of complex samples containing a fairly low amount of peptide material derived from Salmonella, therefore enabling simultaneous host and pathogen protein expression profiling reflecting actual infection conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:18:17.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ursula Fels
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-09 06:25:09	ID requested
1	2021-01-28 02:40:12	announced
⏵ 2	2024-10-22 05:18:21	announced	2024-10-22: Updated project metadata.

Willems P, Fels U, Staes A, Gevaert K, Van Damme P, Use of Hybrid Data-Dependent and -Independent Acquisition Spectral Libraries Empowers Dual-Proteome Profiling. J Proteome Res, 20(2):1165-1177(2021) [pubmed]

10.1021/acs.jproteome.0c00350;

submitter keyword: data-dependent acquisition (DDA),Proteomics, Salmonella, spectral library, data-independent acquisition (DIA)

Petra Van Damme
contact affiliation	Department of Biochemistry and Microbiology, Ghent University, Belgium
contact email	Petra.VanDamme@ugent.be
lab head
Ursula Fels
contact affiliation	UGent
contact email	ursulafels@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD017945

PRIDE project URI

• PRIDE
  1. PXD017945
     1. Label: PRIDE project
     2. Name: Data-dependent and independent acquisition of complex human-Salmonella mixed proteomes