PXD017932-1

PXD017932 is an original dataset announced via ProteomeXchange.

Title	Identification and quantification of small proteins and peptides in Staphylococcus aureus
Description	Very small proteins with less than 100 amino acids (SP100) of Staphylococcus aureuse are underrepresented in proteomic analyses so far. However, in the last few years a variety of such small proteins with regulatory and virulence associated functions have been detected in several bacteria. The introduction of a new open source command line tool (Pepper) that provides a fully automated proteogenomic workflow enabled us to identify proteins encoded by non-annotated open reading frames based on identified peptides. Altogether, 185 soluble proteins with up to 100 amino acids have been detected of which 69 were not covered by the used gene annotation. Of these, 83 % were identified by at least two methods.
HostingRepository	PRIDE
AnnounceDate	2021-05-26
AnnouncementXML	Submission_2021-05-26_06:27:34.691.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kucklick
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-09 02:02:26	ID requested
⏵ 1	2021-05-26 06:27:35	announced
2	2024-10-22 05:22:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Staphylococcus aureus, small proteins, SP100, proteogenomics

Susanne Engelmann
contact affiliation	Mikrobielle Proteomik, Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany
contact email	Susanne.Engelmann@helmholtz-hzi.de
lab head
Martin Kucklick
contact affiliation	TU Braunschweig Institut für Mikrobiologie Spielmannstr. 7 Germany
contact email	m.kucklick@tu-braunschweig.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD017932

PRIDE project URI

• PRIDE
  1. PXD017932
     1. Label: PRIDE project
     2. Name: Identification and quantification of small proteins and peptides in Staphylococcus aureus