PXD017901 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative Proteomics of different truffle species |
Description | Truffles of the Tuber species as expensive foods are predestined for food fraud. To find markers for distinguishing different truffle species the proteomes as phenotype determining basis were investigated. First, established sample preparation protocols subsequent to LC-MS/MS analysis for a bottom up proteomic approach were tested on a truffle sample. The sample preparation included protein extraction and in-solution tryptic digestion. For protein extraction sodium deoxycholate (SDC), sodium dodecylsulfate (SDS) and urea were tested, followed by direct tryptic digestion in solution (SDC extracted proteins) and a tryptic digestion utilizing the FASP approach with molecular cut-off filters (SDS and urea extracted proteins). By this approach the SDC sample preparation protocol was classified as the most suited one to prepare truffle samples. It resulted in a high protein identification rate, had a high reproducibility, was cost efficient and most easy to handle. By a quantitative proteomic approach using label-free and non-targeted bottom up LC-MS/MS the species T. magnatum (n=13), T. aestivum (n=16), T. uncinatum (n=4) and T. melanosporum (n=7) were compared and truffle species specific markers were identified. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:30:43.642.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dennis Krösser |
SpeciesList | scientific name: Tuber melanosporum Mel28; NCBI TaxID: 656061; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-05 04:35:45 | ID requested | |
1 | 2021-12-18 23:14:46 | announced | |
⏵ 2 | 2024-10-22 05:30:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, truffle species,truffle, food profiling, LC-MS/MS |
Contact List
Hartmut Schlüter |
contact affiliation | Universiy Medical Center Hamburg-Eppendorf, Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics |
contact email | hschluet@uke.de |
lab head | |
Dennis Krösser |
contact affiliation | University Medical Center Hamburg-Eppendorf |
contact email | d.kroesser@uke.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017901
- Label: PRIDE project
- Name: Quantitative Proteomics of different truffle species