PXD017869-1

PXD017869 is an original dataset announced via ProteomeXchange.

Title	Affinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi
Description	Chromatin remodeling is a general mechanism involved in DNA-dependent processes, including gene expression regulation. ISWI ATPase is the catalytic subunit in diverse chromatin remodeling complexes that changing histone–DNA interactions and therefore play a pivotal role in different nuclear processes. Here, the ISWI remodeler was fused to GFP (NH and CO fusions) and its partners were determined by affinity purification-mass spectrometry (MS) assay. This approach identified known ISWI partners (NLP, RCCP and FYRP), previously characterized in T. brucei, and new components of TcISWI complex (DRBD2, DHH1 and proteins containing SMC domains).
HostingRepository	PRIDE
AnnounceDate	2020-05-25
AnnouncementXML	Submission_2020-05-25_07:41:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 02:05:22	ID requested
⏵ 1	2020-05-25 07:41:44	announced
2	2024-10-22 05:04:25	announced	2024-10-22: Updated project metadata.

D, í, az-Olmos Y, Batista M, Ludwig A, Marchini FK, Characterising ISWI chromatin remodeler in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, 115():e190457(2020) [pubmed]

submitter keyword: Trypanosoma cruzi, LC-MS/MS, affinity purification, ISWI

Fabricio Klerynton Marchini
contact affiliation	Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil
contact email	fabricio.marchini@fiocruz.br
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017869
     1. Label: PRIDE project
     2. Name: Affinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi