PXD017836-1

PXD017836 is an original dataset announced via ProteomeXchange.

Title	Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus
Description	Mineralised dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. In this study we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of 16th century human dental calculus from an individual from Trondheim, Norway. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. M. leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual’s dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_00:46:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Fotakis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 23:38:54	ID requested
⏵ 1	2020-10-19 00:46:49	announced

Fotakis AK, Denham SD, Mackie M, Orbegozo MI, Mylopotamitaki D, Gopalakrishnan S, Sicheritz-Pont, é, n T, Olsen JV, Cappellini E, Zhang G, Christophersen A, Gilbert MTP, V, å, gene Å J, in archaeological human dental calculus. Philos Trans R Soc Lond B Biol Sci, 375(1812):20190584(2020) [pubmed]

submitter keyword: palaeoproteomics, dental calculus

Thomas P. Gilbert
contact affiliation	Section for Evolutionary Genomics, Globe Insitute, University of Copenhagen
contact email	tgilbert@sund.ku.dk
lab head
Anna Fotakis
contact affiliation	University of Copenhagen
contact email	anna.fotakis@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017836
     1. Label: PRIDE project
     2. Name: Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus