PXD017828 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum |
Description | The archaea Cuniculiplasma divulgatum are abundant in acidic environments with low to moderate temperatures. However, the molecular mechanisms underlying its ability to thrive at low temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold shock. The C. divulgatum genome encodes 2,016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of C. divulgatum, its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock (the core proteome), whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in protein levels was found for the metal-dependent hydrolase, FAD-dependent oxidoreductase, aspartate carbamoyltransferase regulatory chain proteins, 2-oxoacid ferredoxin oxidoreductase and ATPase-V type ATP synthase. Furthermore, the cold shock induced a substantial increase (3.5 % and 9.0 %) in the levels of two most abundant intracellular proteins, chaperonin and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of Cuniculiplasma spp. allowing them to colonise and survive in acidic settings at low/ moderate temperatures. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-26 |
AnnouncementXML | Submission_2020-05-26_06:45:03.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD017828 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rafael Bargiela |
SpeciesList | scientific name: Cuniculiplasma divulgatum; NCBI TaxID: 1673428; |
ModificationList | Ammonia-loss; Deamidated; Dehydrated; Oxidation; deaminated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | SYNAPT G2-Si; nanoACQUITY UPLC |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-02 06:08:42 | ID requested | |
⏵ 1 | 2020-05-26 06:45:04 | announced | |
Publication List
Bargiela R, Lanthaler K, Potter CM, Ferrer M, Yakunin AF, Paizs B, Golyshin PN, Golyshina OV, . Microorganisms, 8(5):(2020) [pubmed] |
Keyword List
submitter keyword: Acidophilic archaea, acidic environments, acid mine drainage systems, Thermoplasmatales, Cuniculiplasma, proteome, cold shock |
Contact List
Peter Golyshin |
contact affiliation | Centre for Environmental Biotechnology, Bangor University, Deiniol Rd, Bangor, LL57 2UW, UK School of Natural Sciences, Bangor University, Deiniol Rd, Bangor, LL57 2UW, UK |
contact email | p.golyshin@bangor.ac.uk |
lab head | |
Rafael Bargiela |
contact affiliation | Center for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor |
contact email | f.bargiela@bangor.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017828
- Label: PRIDE project
- Name: Proteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum