PXD017732-1

PXD017732 is an original dataset announced via ProteomeXchange.

Title	Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium
Description	In the current study we examined several proteomic- and RNA-Seq-based datasets of cardiac-enriched, cell-surface and membrane-associated proteins in human fetal and mouse neonatal ventricular cardiomyocytes. By integrating available microarray and tissue expression profiles along with MGI phenotypic analysis, we identified 173 membrane-associated proteins that are cardiac-enriched, conserved amongst eukaryotic species, and have not yet been linked to a ‘cardiac’ Phenotype-Ontology. To highlight the utility of this dataset, we selected several proteins to investigate more carefully, including FAM162A, MCT1, and COX20, to show cardiac enrichment, subcellular distribution and expression patterns in disease. Three-dimensional imaging was used to validate subcellular localization and expression in adult mouse ventricular cardiomyocytes. FAM162A, MCT1, and COX20 were differentially expressed at the transcriptomic and proteomic levels in multiple models of mouse and human heart diseases and may represent potential diagnostic and therapeutic targets for human dilated and ischemic cardiomyopathies. Altogether, we believe this comprehensive cardiomyocyte membrane proteome dataset will prove instrumental to future investigations aimed at characterizing heart disease markers and/or therapeutic targets for heart failure.
HostingRepository	PRIDE
AnnounceDate	2020-11-17
AnnouncementXML	Submission_2020-11-17_08:44:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Shin-Haw Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-26 02:44:06	ID requested
⏵ 1	2020-11-17 08:44:50	announced
2	2024-10-22 05:15:14	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Membrane proteins, Cardiomyocytes, Bioinformatics, Heart disease, Therapeutics

Anthony Gramolini
contact affiliation	University of Toronto Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research
contact email	anthony.gramolini@utoronto.ca
lab head
Frank Shin-Haw Lee
contact affiliation	University of Toronto
contact email	shinhaw.lee@mail.utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD017732

PRIDE project URI

• PRIDE
  1. PXD017732
     1. Label: PRIDE project
     2. Name: Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium