PXD017722-1

PXD017722 is an original dataset announced via ProteomeXchange.

Title	A strain-specific genomic island encodes regulatory proteins to activate type VI secretion system expression in the squid symbiont Vibrio fischeri.
Description	We used the bioluminescent squid symbiont, Vibrio fischeri, to identify essential regulatory factors that control expression and function of a strain-specific T6SS encoded within a genomic island. Random transposon mutagenesis revealed that three genes located on the T6SS2-encoding genomic island are necessary to activate expression of a T6SS reporter. We used a proteomics approach to identify proteins that were differentially abundant in mutant strains compared to the wild type when cells were grown in a high-viscosity media.
HostingRepository	PRIDE
AnnounceDate	2021-08-24
AnnouncementXML	Submission_2021-08-24_03:11:53.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernanda Salvato
SpeciesList	scientific name: Aliivibrio fischeri; NCBI TaxID: 668;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-25 07:23:27	ID requested
⏵ 1	2021-08-24 03:11:53	announced
2	2024-10-22 05:24:49	announced	2024-10-22: Updated project metadata.

Smith S, Salvato F, Garikipati A, Kleiner M, Septer AN, Requires the Transcriptional Regulator TasR and the Structural Proteins TssM and TssA. J Bacteriol, 203(21):e0039921(2021) [pubmed]

submitter keyword: 1D-LC-MS/MS, Aliivibrio fischeri, Vibrio fischeri, Squid-Vibrio symbiosis, Type VI secretion system, symbiosis, genomic island

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Fernanda Salvato
contact affiliation	North Carolina State University
contact email	fersalvato@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD017722

PRIDE project URI

• PRIDE
  1. PXD017722
     1. Label: PRIDE project
     2. Name: A strain-specific genomic island encodes regulatory proteins to activate type VI secretion system expression in the squid symbiont Vibrio fischeri.