PXD017721-1

PXD017721 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling of KAIMRC1 in comparison to MDA-MB231 and MCF-7
Description	Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF7 and MDA-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT) labeled technique in conjunction with phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, Splicing machinery, amino acid metabolism, cellular energy, and Estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including MTAP, KCTD12, PARP 1, and Prelamin-A/C were subjected to western blotting and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.
HostingRepository	PRIDE
AnnounceDate	2020-06-22
AnnouncementXML	Submission_2020-06-21_22:24:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bandar Alghanem
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-25 07:21:15	ID requested
⏵ 1	2020-06-21 22:24:19	announced

Dataset with its publication pending

submitter keyword: Breast cancer

cell lines

TMT

Proteomics

Phosphoproteomics

Mohamed Boudjelal
contact affiliation	King Abdullah international medical research center
contact email	boudjelalmo@ngha.med.sa
lab head
Bandar Alghanem
contact affiliation	king abdullah international medical research center
contact email	ghanembandar@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017721

PRIDE project URI

• PRIDE
  1. PXD017721
     1. Label: PRIDE project
     2. Name: Proteomics profiling of KAIMRC1 in comparison to MDA-MB231 and MCF-7