PXD017708-2

PXD017708 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals rapid divergence in the postmating response of female reproductive tracts among sibling species
Description	Fertility depends, in part, on interactions between male and female reproductive proteins inside the female reproductive tract (FRT) that mediate postmating changes in female behavior, morphology, and physiology. Coevolution between interacting proteins within species may drive reproductive incompatibilities between species, yet the mechanisms underlying postmating-prezygotic isolating barriers remain poorly resolved. Here, we used quantitative proteomics in sibling Drosophila species to investigate the molecular composition of the FRT environment and its role in mediating species-specific postmating responses. We found that (1) FRT proteomes in D. simulans and D. mauritiana virgin females express unique combinations of secreted proteins and are enriched for distinct functional categories, (2) mating induces substantial changes to the FRT proteome in D. mauritiana but not in D. simulans, and (3) the D. simulans FRT proteome exhibits limited postmating changes irrespective of whether females mate with conspecific or heterospecific males, suggesting an active female role in mediating reproductive interactions. Our study suggests that divergence in the FRT extracellular environment and postmating response contribute to previously described patterns of postmating-prezygotic isolation and the maintenance of species boundaries.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:48.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erin McCullough
SpeciesList	scientific name: Drosophila simulans; NCBI TaxID: 7240; scientific name: Drosophila mauritiana; NCBI TaxID: 7226;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-24 22:46:29	ID requested
1	2020-06-25 04:50:28	announced
⏵ 2	2024-10-22 05:07:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Drosophila simulans, LC-MSMS, female reproductive tract, TMT, Drosophila mauritiana

Steve Dorus
contact affiliation	Center for Reproductive Evolution, Biology Department, Syracuse University
contact email	sdorus@syr.edu
lab head
Erin McCullough
contact affiliation	Syracuse University
contact email	mccullough.e@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017708

PRIDE project URI

• PRIDE
  1. PXD017708
     1. Label: PRIDE project
     2. Name: Quantitative proteomics reveals rapid divergence in the postmating response of female reproductive tracts among sibling species