PXD017699-2

PXD017699 is an original dataset announced via ProteomeXchange.

Title	Cul4b promotes CD4 T cell expansion by aiding the repair of damaged DNA
Description	The capacity for T cells to become activated and clonally expand during pathogen invasion is pivotal for protective immunity. Our understanding of how T cell receptor (TCR) signaling prepares cells for this rapid expansion remains limited. We have identified an E3 ubiquitin ligase, Cul4b, that regulates this process. Cul4b levels and activity increase following TCR stimulation. Disruption of Cul4b resulted in impaired proliferation and survival of activated T cells. Additionally, Cul4b-deficient CD4 T cells accumulated DNA damage. In T cells, Cul4b preferentially associated with the substrate receptor DCAF1, and Cul4b and DCAF1 were found to interact with proteins that promote the sensing or repair of damaged DNA. While Cul4b-deficient CD4 T cells showed evidence of DNA damage sensing, downstream phosphorylation of SMC1A did not occur. These findings reveal an essential role for Cul4b in promoting the repair of damaged DNA to allow survival and expansion of activated T cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:11.887.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-24 02:22:48	ID requested
1	2021-01-04 01:22:59	announced
⏵ 2	2024-10-22 05:17:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DCAF1, CRL4, E3 ubiquitin ligase,T Cell Receptor, Apoptosis, SMC1A, Cul4b, Cell Cycle, T cells, Proliferation, adaptive immune response, DNA damage response

Steven H. Seeholzer
contact affiliation	Director, Protein and Proteomics Core Children's Hospital of Philadelphia Research Institute
contact email	seeholzer@email.chop.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD017699

PRIDE project URI

• PRIDE
  1. PXD017699
     1. Label: PRIDE project
     2. Name: Cul4b promotes CD4 T cell expansion by aiding the repair of damaged DNA