PXD017696-1

PXD017696 is an original dataset announced via ProteomeXchange.

Title	Comprehensive quantitative analysis of the proteome of Leishmania (Viannia) species
Description	Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomic analyses did not follow these achievements and Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of L. braziliensis, L. panamensis and L. guyanensis. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. “Total Protein Approach” and “Proteomic Ruler” were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most complete Leishmania proteome yet known, and provides a quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. We describe the abundance of proteins from the major energy metabolic processes, highlighting the main differences among the species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which delineate significant differences among those species and provide rich substrate for explore new molecules for diagnosing purposes.
HostingRepository	PRIDE
AnnounceDate	2020-08-10
AnnouncementXML	Submission_2020-08-09_23:12:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Leishmania guyanensis species complex; NCBI TaxID: 38579;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-24 00:33:06	ID requested
⏵ 1	2020-08-09 23:12:12	announced

Dataset with its publication pending

submitter keyword: Leishmania (Viannia)

Leishmania braziliensis

Leishmania panamensis

Leishmania guyanensis

proteomics

label-free quantification

Filter Aided Sample Preparation, Total Protein Approach, Proteomic Ruler, mass spectrometry

Jacek R Wisniewski
contact affiliation	Max Planck Institute of Biochemistry Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Max Planck Institute of Biochemistry Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017696

PRIDE project URI

• PRIDE
  1. PXD017696
     1. Label: PRIDE project
     2. Name: Comprehensive quantitative analysis of the proteome of Leishmania (Viannia) species