PXD017677-1

PXD017677 is an original dataset announced via ProteomeXchange.

Title	SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
Description	Neural stem cell regulation is essential for the formation of the central nervous system and homeostatic neurogenesis in the adult mammalian brain. The RNAseIII Drosha, a key component of the miRNA microprocessor, plays a central role in regulating NSC maintenance partially through a miRNA-independent mechanism. Drosha controls mRNA expression levels by targeting and cleaving evolutionary conserved stem-loop hairpins located in the mRNAs of stem cell-related transcription factors. However, it is unknown how the Drosha-mediated endonucleolytic cleavage of mRNA is regulated. Here, we identify novel Drosha and NFIB interactors in hippocampal NSCs by in vitro pull-down assays followed by Mass Spectrometry. We unravel the RNA binding proteins implicated in Drosha-mediated regulation of neurogenesis and we find Scaffold Attachment Factor B1 to play a novel and essential role in NFIB mRNA regulation during neural stem cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2024-06-03
AnnouncementXML	Submission_2024-06-03_05:57:30.305.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017677
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Bock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-21 02:44:52	ID requested
⏵ 1	2024-06-03 05:57:30	announced

10.6019/PXD017677;

submitter keyword: Neural Stem Cell,Drosha

Alexander Schmidt
contact affiliation	University of Basel, Biozentrum, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Thomas Bock
contact affiliation	Proteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact email	thomas.bock@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017677
     1. Label: PRIDE project
     2. Name: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA