PXD017622-1

PXD017622 is an original dataset announced via ProteomeXchange.

Title	A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission.
Description	Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. We also provide evidence for indirect regulation of the concomitant M-phase progression. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this unique cyclin/CDK pair fills the functional space of multiple eukaryotic cell-cycle kinases controlling DNA replication and M-phase progression.
HostingRepository	PRIDE
AnnounceDate	2020-06-17
AnnouncementXML	Submission_2020-06-16_23:01:48.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-19 07:43:16	ID requested
⏵ 1	2020-06-16 23:01:48	announced

Dataset with its publication pending

submitter keyword: Malaria, Plasmodium, Cyclin, Cyclin-Dependent, kinase, parasite, Warwick-RTP

Dr. Rita Tewari
contact affiliation	School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH
contact email	rita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017622
     1. Label: PRIDE project
     2. Name: A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission.