PXD017542-1

PXD017542 is an original dataset announced via ProteomeXchange.

Title	Production and application of recombinant extracellular vesicles for method development, data normalization and assessment of pre-analytical variables
Description	The diagnostic and therapeutic use of extracellular vesicles (EV) is under intense investigation and may lead to societal benefits. Reference materials are an invaluable resource for developing, improving and assessing the performance of regulated EV applications and for quantitative and objective data interpretation. We have engineered recombinant extracellular vesicles (rEV) as a biological reference material. rEV have similar biochemical and biophysical characteristics as sample EV and function as an internal quantitative and qualitative control throughout analysis. Spike-in applications of rEV in bodily fluids prior to EV analysis map technical variability of EV applications and promote intra and inter laboratory studies. This protocol describes the production, recovery and quality assurance of rEV, their dilution and addition to bodily fluids, and the detection steps based on fluorescence-, nucleic acid- and protein measurements. Multiple application potentials for rEV are exemplified, including method development, big data normalization and assessment of pre-analytical variables. The protocol can be adopted by researchers with standard laboratory and basic EV separation/characterization experience and requires ~4–5 d.
HostingRepository	PRIDE
AnnounceDate	2023-03-13
AnnouncementXML	Submission_2023-03-13_00:11:47.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PekkaRappu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-17 03:05:32	ID requested
⏵ 1	2023-03-13 00:11:47	announced
2	2023-11-14 08:50:53	announced	2023-11-14: Updated project metadata.

submitter keyword: recombinant, urine,extracellular vesicles, human, plasma

Olivier DeWever
contact affiliation	Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium
contact email	Olivier.DeWever@UGent.be
lab head
PekkaRappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD017542

PRIDE project URI

• PRIDE
  1. PXD017542
     1. Label: PRIDE project
     2. Name: Production and application of recombinant extracellular vesicles for method development, data normalization and assessment of pre-analytical variables