PXD017540-1

PXD017540 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus-induced proteomic changes in the mammary tissue of rats: A TMT study
Description	Staphylococcus aureus is one of the most important pathogens causing mastitis in dairy cows. The objective of this study was to establish a rat mastitis model induced by a S.aureus infection and to explore proteins that change in proteomes of mammary tissue in different udders states,which provided a better understanding of the host immune response to S. aureus mastitis. Six 72 h postpartum rats were randomly divided into two groups (n=3), with either 100 µL of PBS (blank group) or a S. aureus suspension containing 2×107 CFU·ml−1 (challenge group) infused into the mammary gland duct,respectively. After 24 h of infection, the rats were sacrificed, and mammary gland tissue was collected. A tandem mass tag (TMT)-based technology was applied to compare the proteomes of healthy and mastitic mammary tissue. Compared with the control group, the challenge group had 555 proteins with significant differences in expression, of which 428 were significantly up-regulated (FC>1.2 and p<0.05) and 127 were down-regulated (FC>0.83 and p<0.05 or p<0.01). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that up-regulated differentially significant expressed proteins (DSEPs) were associated with mainly immune responses,including integrin alpha M,inter-α-trypsin inhibitor heavy chain 4,alpha-2-macroglobulin.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:02:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lirong Lirong
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-17 03:05:09	ID requested
⏵ 1	2020-05-26 16:02:35	announced
2	2024-10-22 05:05:51	announced	2024-10-22: Updated project metadata.

Cai L, Tong J, Zhang Z, Zhang Y, Jiang L, Hou X, Zhang H, Staphylococcus aureus-induced proteomic changes in the mammary tissue of rats: A TMT-based study. PLoS One, 15(5):e0231168(2020) [pubmed]

submitter keyword: Staphylococcus aureus，proteomic，mastitis

Beijing Key Laboratory of Dairy Cow Nutrition
contact affiliation	Beijing University of Agriculture, China
contact email	huazhang0914@163.com
lab head
Lirong Lirong
contact affiliation	Lirong
contact email	clr31415926@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017540
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus-induced proteomic changes in the mammary tissue of rats: A TMT study