PXD017525-1

PXD017525 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of chemical and genetic EZH2 inhibition in diffuse intrinsic pontine glioma cell lines
Description	Diffuse Intrinsic Pontine Glioma (DIPG) is a rare and highly aggressive pediatric tumor. The average survival time after diagnosis is less than one year. Currently, there are no effective treatments. Characteristic of DIPG is a mutation in histone H3 which leads to a substitution of Lysine 27 to Methionine (H3K27M) which deregulates Polycomb Repressive Complex 2 (PRC2), including enzymatic activity of EZH2. Previous studies have shown that inhibition of EZH2 by chemical agents decreases DIPG cell proliferation and inhibits tumor growth in vivo. My thesis project aims to confirm that EZH2 could be a therapeutic target using chemical EZH2 inhibitors, small interfering RNAs and a CRISPR/Cas9 approach in a series of DIPG tumor cell lines and to determine underlying molecular mechanisms of action.
HostingRepository	PRIDE
AnnounceDate	2022-04-01
AnnouncementXML	Submission_2022-04-01_10:35:24.000.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 08:55:15	ID requested
⏵ 1	2022-04-01 10:35:24	announced
2	2024-10-22 05:35:45	announced	2024-10-22: Updated project metadata.

Rahal F, Capdevielle C, Rousseau B, Izotte J, Dupuy JW, Cappellen D, Chotard G, M, é, nard M, Charpentier J, Jecko V, Caumont C, Gimbert E, Grosset CF, Hagedorn M, An EZH2 blocker sensitizes histone mutated diffuse midline glioma to cholesterol metabolism inhibitors through an off-target effect. Neurooncol Adv, 4(1):vdac018(2022) [pubmed]

submitter keyword: Diffuse intrinsic pontine glioma, quantitative proteome, EZH2 inhibition, GSK126, EZH2 knock down

Martin Hagedorn
contact affiliation	INSERM U1035 – BMGIC, Univ Bordeaux, 146 rue Leo Saignat, F-33000 Bordeaux, France.
contact email	martin.hagedorn@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD017525

PRIDE project URI

• PRIDE
  1. PXD017525
     1. Label: PRIDE project
     2. Name: Proteomic analysis of chemical and genetic EZH2 inhibition in diffuse intrinsic pontine glioma cell lines