PXD017496-2

PXD017496 is an original dataset announced via ProteomeXchange.

Title	Global and site-specific effect of phosphorylation on protein turnover
Description	The effects of specific modification types and sites on protein lifetime have not been illustrated in large scale. We describe a proteomic method, DeltaSILAC, to quantify the impact of site-specific phosphorylation on the turnover of thousands of proteins in live cells. Being configured on the accurate and reproducible mass spectrometry, the pulse labeling approach using stable isotope-labeled amino acids in cells (pSILAC), phosphoproteomics, as well as a novel peptide-level matching strategy, our DeltaSILAC profiling revealed a global, unexpected delaying effect of many phosphosites on protein turnover. We further found that phosphorylated sites accelerating protein turnover are functionally selected for cell fitness, enriched in Cyclin-dependent kinase substrates, and evolutionarily conserved, whereas the Glutamic acids surrounding phosphosites significantly delay protein turnover. Our investigation provides a generalizable approach and a rich resource for prioritizing the effects of phosphorylation sites on protein lifetime in the context of cell signaling and disease biology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:12.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yansheng Liu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-13 02:40:20	ID requested
1	2020-11-17 06:19:57	announced
⏵ 2	2024-10-22 05:15:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Phosphoproteomics,Cell line, DIA, Turnover, Lumos

Yansheng Liu
contact affiliation	Yale University School of Medicine
contact email	yansheng.liu@yale.edu
lab head
Yansheng Liu
contact affiliation	Yale University School of Medicine
contact email	yansheng.liu@yale.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017496
     1. Label: PRIDE project
     2. Name: Global and site-specific effect of phosphorylation on protein turnover