PXD017470-1

PXD017470 is an original dataset announced via ProteomeXchange.

Title	Ferrosomes are iron storage organelles formed by broadly conserved gene clusters in bacteria and archaea
Description	Cellular iron homeostasis is vital and maintained through tight regulation of iron import, efflux, storage, and detoxification. The most common modes of iron storage employ proteinaceous compartments that are composed of ferritin or related proteins. While lipid-bounded iron compartments have also been described, the basis for their formation and function remains unknown. Here, we focus on one such compartment, the ferrosome, which had been previously observed in the anaerobic bacterium Desulfovibrio magneticus. We identify three ferrosome-associated (Fez) proteins, encoded by a putative operon, that are associated with and responsible for forming ferrosomes in D. magneticus. Fez proteins include FezB, a P1B-6-ATPase found in phylogenetically and metabolically diverse species of bacteria and archaea with anaerobic lifestyles. In the majority of these species, two to ten genes define a cluster that encodes FezB. We show that two other species, Rhodopseudomonas palustris and Shewanella putrefaciens, make ferrosomes in anaerobic conditions through the action of their six-gene fez operon. Additionally, we find that the S. putrefaciens fez operon is sufficient for ferrosome formation in Escherichia coli. Using S. putrefaciens as a model, we find that ferrosomes likely play a role in the anaerobic adaptation to iron starvation. Overall, this work establishes ferrosomes as a new class of lipid-bounded iron storage organelles and sets the stage for studying ferrosome formation and structure in diverse microorganisms.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_03:33:10.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carly Grant
SpeciesList	scientific name: Desulfovibrio magneticus RS-1; NCBI TaxID: 573370;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt G2-S HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 03:00:42	ID requested
⏵ 1	2022-05-31 03:33:10	announced
2	2024-10-22 05:37:21	announced	2024-10-22: Updated project metadata.

Grant CR, Amor M, Trujillo HA, Krishnapura S, Iavarone AT, Komeili A, Distinct gene clusters drive formation of ferrosome organelles in bacteria. Nature, 606(7912):160-164(2022) [pubmed]

submitter keyword: bacteria, organelle, anaerobic, iron

Arash Komeili
contact affiliation	Department of Plant and Microbial Biology, University of California, Berkeley, USA
contact email	komeili@berkeley.edu
lab head
Carly Grant
contact affiliation	University of California, Berkeley
contact email	carly.grant@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD017470

PRIDE project URI

• PRIDE
  1. PXD017470
     1. Label: PRIDE project
     2. Name: Ferrosomes are iron storage organelles formed by broadly conserved gene clusters in bacteria and archaea