PXD017462-2

PXD017462 is an original dataset announced via ProteomeXchange.

Title	Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants
Description	The proteome of two sperm sources, epididymal and ejaculated sperm, was compared and associated with sperm freezing capacity using samples collected from three wild small ruminants species. Protein identification was performed using two databases separately (Ovis aries and Capra hircus NCBI) and results were later combined.
HostingRepository	PRIDE
AnnounceDate	2021-05-05
AnnouncementXML	Submission_2021-05-04_22:16:03.903.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017462
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Sylvester
SpeciesList	scientific name: Rupicapra pyrenaica; NCBI TaxID: 72545; scientific name: Capra pyrenaica; NCBI TaxID: 80419; scientific name: Ovis aries musimon; NCBI TaxID: 9938;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; Propionamide; S-carboxamidoethyl-L-cysteine; TMT6plex; Oxidation; Acetyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 01:42:31	ID requested
1	2021-05-04 03:22:18	announced
⏵ 2	2021-05-04 22:16:04	announced	2021-05-05: Updated project metadata.

10.1016/J.CRYOBIOL.2021.01.012;

Mart, í, nez-Fresneda L, Sylvester M, Shakeri F, Bunes A, Del Pozo JC, Garc, í, a-V, á, zquez FA, Neuhoff C, Tesfaye D, Schellander K, Santiago-Moreno J, Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants. Cryobiology, 99():64-77(2021) [pubmed]

submitter keyword: germplasm bank, proteomics, TMT, mass spectrometry, sperm, goat, sheep, cross-species,cryopreservation

Julian Santiago-Moreno
contact affiliation	Department of Animal Reproduction, Spanish National Institute for Agricultural and Food Research and Technology (INIA), Avda. Puerta de Hierro km 5.9, 28040 Madrid, Spain.
contact email	moreno@inia.es
lab head
Marc Sylvester
contact affiliation	Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn
contact email	msylvest@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD017462

PRIDE project URI

• PRIDE
  1. PXD017462
     1. Label: PRIDE project
     2. Name: Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants