PXD017458-1

PXD017458 is an original dataset announced via ProteomeXchange.

Title	Phospho-proteomic Profiling Dataset of Chemical Perturbations in Multiple Biological Backgrounds
Description	The Library of Integrated Network-based Cellular Signatures (LINCS) Program aims to create a network-based understanding of biology by cataloging changes in gene expression and other cellular processes that occur when cells are exposed to a variety of perturbing agents. The goal of this project is to test the hypothesis that modulation of phosphorylation-mediated signaling events in response to perturbations can establish new cellular states by altering their epigenetic landscape. We have performed mass spectrometry (MS)-based proteomic assays that target quantitative readouts of phospho-signaling and chromatin modifications in cellular models, following perturbation by compounds with varying mechanisms of action. We have employed 128 compounds and treated 5 cancer and 2 neuronal cell lines. The resulting data collected and tools created have been contributed to the LINCS program for the purpose of making connections among disparate perturbations through phosphoproteomics and chromatin modification signatures in concert with other available data types. The data is publicly available on PanoramaPublic and can be queried using Clue (Broad Institute). Analysis of our data will inform novel therapeutic opportunities and synergies, as dysregulation of phospho-signaling and epigenetic systems are two of the most common molecular etiologies identified in a growing number of diseases.
HostingRepository	PanoramaPublic
AnnounceDate	2020-08-14
AnnouncementXML	Submission_2020-08-14_15:51:46.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karen Christianson
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Acetyl; Phospho; Oxidation; Label:13C(6)15N(4); Label:13C(6)15N(2); Label:13C(6)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-11 13:55:12	ID requested
⏵ 1	2020-08-14 15:51:46	announced
2	2022-03-02 15:58:57	announced	: Added PubMed Id

Dataset with its publication pending

submitter keyword: Data-Independent Acquisition, mass spectrometry, proteomics, phospho-signaling, phosphoproteomics

Malvina Papanastasiou
contact affiliation	Broad Institute of MIT and Harvard
contact email	malpap@broadinstitute.org
lab head
Karen Christianson
contact affiliation	Broad Institute of MIT and Harvard
contact email	karen@broadinstitute.org
dataset submitter

Panorama Public dataset URI