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PXD017416-2

PXD017416 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimised Workflow for the Proteomic Detection of Small Proteins
DescriptionSmall open reading frame encoded proteins (SEPs) gained increasing interest during the last years due to their broad range of important functions in both, prokaryotes and in eukaryotes. In bacteria, signalling, virulence or regulation of enzyme activities have been associated with this protein class. Nonetheless, the number of SEPs detected in large scale proteome studies is often low as classical methods are biased towards the identification of larger proteins. In the accompanying manuscript, we present a workflow that allows enhanced identification rates of small proteins compared to traditional protocols. For this aim, the steps of small protein enrichment, proteolytic digest and database search were reviewed and adjusted to the special requirement of SEPs. Enrichment by the use of small pore sized solid phase material increased the absolute numbers of identified SEPs by a factor of two and the application of alternative proteases to trypsin reduced spectral counts for larger proteins. The application of an integrated proteogenomics search database (iPtgxDB) in combination with the optimized protocol allowed the detection of several proteins not yet annotated in the reference genome of Bacillus subtilis. Finally, the identification of some of these missed proteins was validated by comparing their identifying spectra to those of synthetic peptides.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:14.026.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuergen Bartel
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationListcarbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-06 04:14:18ID requested
12020-10-12 07:13:16announced
22024-10-22 05:13:19announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.0c00286;
Bartel J, Varadarajan AR, Sura T, Ahrens CH, Maa, ß S, Becher D, Optimized Proteomics Workflow for the Detection of Small Proteins. J Proteome Res, 19(10):4004-4018(2020) [pubmed]
Keyword List
curator keyword: Technical, Biological
submitter keyword: database search, small protein enrichment, SEPs,proteogenomics, Bacillus subtilis, sProteins, proteolytic digest
Contact List
Dörte Becher
contact affiliationUniversity of Greifswald, Institute for Microbiology, Department for Microbial Proteomics
contact emaildbecher@uni-greifswald.de
lab head
Juergen Bartel
contact affiliationUniversity of Greifswald, Department for Microbial Proteomics
contact emailjuergen.bartel@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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