PXD017415-1

PXD017415 is an original dataset announced via ProteomeXchange.

Title	Isobaric labeling strategy utilizing 4-plex N, N-dimethyl leucine (DiLeu) tags reveals proteomic changes induced by chemotherapy-related neurotoxicity in cerebrospinal fluid of children
Description	Using mass spectrometry for protein identification and quantification in cerebrospinal fluid (CSF) is at the forefront of research efforts to explore the pathophysiology biomarkers for the early diagnosis and prognosis of neurologic disorders. Here we implemented 4-plex N, N-dimethyl leucine (DiLeu) isobaric labeling reagents in a longitudinal study to quantify CSF proteins from children with B-cell acute lymphoblastic leukemia (B-cell ALL) undergoing chemotherapy. Results from this study identified differentially expressed proteins in CSF samples collected from week 5, week 10-14 and week 24-28 compared with samples before Chemo treatment. 84 proteins were significantly altered while Gene Ontology analysis revealed their involvement in various biological processes associated with neurologic disorders. Results from this study will be helpful for monitoring central nervous system (CNS)-specific proteins and developing preventive strategies for CNS injury in response to Chemo.
HostingRepository	PRIDE
AnnounceDate	2020-07-02
AnnouncementXML	Submission_2020-07-02_02:29:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qinying Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-06 03:44:34	ID requested
⏵ 1	2020-07-02 02:29:59	announced
2	2024-10-22 05:08:18	announced	2024-10-22: Updated project metadata.

Yu Q, Zhong X, Chen B, Feng Y, Ma M, Diamond CA, Voeller JS, Kim M, DeSantes KB, Capitini CM, Patel NJ, Hoover-Regan ML, Burke MJ, Janko K, Puccetti DM, Ikonomidou C, Li L, -Dimethyl Leucine (DiLeu) Tags Reveals Proteomic Changes Induced by Chemotherapy in Cerebrospinal Fluid of Children with B-Cell Acute Lymphoblastic Leukemia. J Proteome Res, 19(7):2606-2616(2020) [pubmed]

submitter keyword: Isobaric labeling, CSF Proteomics, Leukemia

Lingjun Li
contact affiliation	Lingjun Li Lab, School of Pharmacy, University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Qinying Yu
contact affiliation	University of Wisconsin-Madison
contact email	qyu49@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017415

PRIDE project URI

• PRIDE
  1. PXD017415
     1. Label: PRIDE project
     2. Name: Isobaric labeling strategy utilizing 4-plex N, N-dimethyl leucine (DiLeu) tags reveals proteomic changes induced by chemotherapy-related neurotoxicity in cerebrospinal fluid of children