PXD017398-1

PXD017398 is an original dataset announced via ProteomeXchange.

Title	MaTAR25 RNA Antisense Pulldown and Mass Spectrometry
Description	It has been suggested that lncRNAs can interact with transcriptional regulators/co-factors to form ribonucleoprotein (RNP) complexes to regulate the expression of downstream genes in the cell nucleus. To identify potential interacting proteins of MaTAR25, we used two different paired sets of biotin-labeled antisense oligonucleotides targeting MaTAR25 for native RNA antisense oligonucleotide pull-down (RAP) in 4T1 cells followed by qRT-PCR to assess pull-down efficiency. Samples were also eluted from beads for mass spectrometry isobaric tags for relative and absolute quantitative (MS-iTRAQ) analysis to identify proteins that bind to MaTAR25, and PPIB as the corresponding control. We ranked the candidate interactors based on detectable peptides above background in both pair sets of oligonucleotide pull-downs, and selected candidates with at least 2-fold enrichment compared to corresponding PPIB oligo pull-down.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-23_01:53:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kung-Chi Chang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-05 04:12:53	ID requested
⏵ 1	2020-12-23 01:53:19	announced

Dataset with its publication pending

submitter keyword: Mouse, lncRNA, MaTAR25, breast cancer, protein interaction, biotin-oligos

David L. Spector
contact affiliation	Cold Spring Harbor Laboratory
contact email	spector@cshl.edu
lab head
Kung-Chi Chang
contact affiliation	Cold Spring Harbor Laboratory
contact email	kchang@cshl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017398
     1. Label: PRIDE project
     2. Name: MaTAR25 RNA Antisense Pulldown and Mass Spectrometry