PXD017376 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of cellular proteins binding to the 3’UTR of Programmed Cell Death 4 mRNA |
Description | The aim of the project is to identify RNA-binding proteins (RBPs) that interact with the 3’ untranslated region (3’UTR) of the programmed cell death 4 (PDCD4) mRNA. PDCD4 is an inflammatory tumor suppressor gene. The translation of PDCD4 mRNA is regulated by multiple RBPs. The aim is to identify RBPs which are critical for regulating PDCD4 mRNA translation by binding to its 3’UTR. For this purpose, a biotinylated PDCD4 3’UTR RNA was incubated with cytoplasmic lysate from MCF7 human breast carcinoma cells and the RNA-protein complexes pulled down by streptavidin affinity chromatography. The proteins were then eluted and resolved by SDS-PAGE. One particular protein band, migrating close to 55 KDa marker was cut out and submitted for proteomic analysis by in gel digestion and mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-11 |
AnnouncementXML | Submission_2020-12-11_00:41:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chhaya Patole |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-04 07:28:28 | ID requested | |
⏵ 1 | 2020-12-11 00:41:55 | announced | |
Publication List
Kumar R, Poria DK, Ray PS, RNA-binding proteins La and HuR cooperatively modulate translation repression of PDCD4 mRNA. J Biol Chem, 296():100154(2021) [pubmed] |
Keyword List
submitter keyword: RNA-protein interaction, RNA-binding protein, 3’ untranslated region, programmed cell death 4, Mass Spectrometry |
Contact List
Professor Partho Sarothi Ray |
contact affiliation | IISER Kolkata Department of Biological Sciences Mohanpur, Dist. Nadia West Bengal, India |
contact email | psray@iiserkol.ac.in |
lab head | |
Chhaya Patole |
contact affiliation | Instem |
contact email | cpatole@instem.res.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD017376 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017376
- Label: PRIDE project
- Name: Identification of cellular proteins binding to the 3’UTR of Programmed Cell Death 4 mRNA