PXD017375 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts treatment on HeLa cells |
Description | Nothapodytes nimmoniana is a natural source of camptothecin, a known anticancer drug. Stem and leaf extracts of Nothapodytes nimmoniana were studied to assess its in-vitro action on cancer progression events in a cervical cancer cell line, the HeLa. As a well-studied ideal model system, the HeLa has been chosen and the effects of extracts on cancer progression events have been traced out. HeLa cells and media samples were analyzed for differentially expressed proteins and metabolites respectively on RRLC-ESI-QTOFMS. The CBFA2T1, cysteine-rich protein 2-binding protein, Zinc finger protein 788, transcription factor RFX3 and angiomotin-like protein 1 were significantly expressed proteins while 3-Hydroxysuberic acid, Indole-3-carboxylic acid, N8-Acetylspermidine, L-Octanoylcarnitine were significantly expressed metabolites. Aminoacyl-tRNA biosynthesis, purine metabolism, and valine, leucine, arginine, proline metabolism were the most prominent pathways. The multi-omics approach has the greatest metabolites and proteins profiling potential hence was applied here to find signatures of N. nimmoniana extracts treatment of cervical cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:39:34.376.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amey Shirolkar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-04 07:16:13 | ID requested | |
⏵ 1 | 2024-10-08 11:39:34 | announced | |
2 | 2024-10-22 05:01:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Nothapodytes nimmoniana (J. Graham), anticancer, proteomics, metabolomics, RRLC-ESI-QTOFMS, HeLa, camptothecin |
Contact List
Sharad Daulatrao Pawar |
contact affiliation | Research Officer, Department of Pharmacology, National Research Institute of Basic Ayurvedic Sciences (NRIBAS), Kothrud, Pune - 411038, Maharashtra, India |
contact email | sd_pawar@yahoo.com |
lab head | |
Amey Shirolkar |
contact affiliation | Laboratory Technician, RAIFR, Pune |
contact email | amey_593@rediffmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017375
- Label: PRIDE project
- Name: Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts treatment on HeLa cells