PXD017366-1

PXD017366 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass spectrometry-based proteomic characterization of cutaneous melanoma ectosomes reveals the presence of cancer-related molecules
Description	Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Due to the development of protocols for their successful isolation, protein content of extracellular vesicles (EVs) (including ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers. The data concerning the proteome of CM-derived EVs is, however, very limited. The present study used shotgun nanoLC-MS/MS approach to profile protein content of ectosomes from primary (WM115 and WM793) and metastatic (WM266-4 and WM1205Lu) CM cell lines. Also, cancer-promoting effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial-mesenchymal transition and angiogenesis. Isolated ectosomes significantly increased proliferation and motility of recipient cells, most likely due to ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.
HostingRepository	MassIVE
AnnounceDate	2020-05-12
AnnouncementXML	Submission_2020-05-12_06:21:08.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jankowska
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Acetyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-03 16:53:22	ID requested
⏵ 1	2020-05-12 06:21:09	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: biomarkers, cutaneous melanoma, ectosomes, extracellular vesicles, metastasis, invasion

submitter keyword: biomarkers, cutaneous melanoma, ectosomes, extracellular vesicles, metastasis, invasion

Contact List

Malgorzata Przybylo
contact affiliation	Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University
contact email	malgorzata.przybylo@uj.edu.pl
lab head
Jankowska
contact affiliation	Jagiellonian University
contact email	urszula.jankowska@uj.edu.pl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084892/