PXD017347-3

PXD017347 is an original dataset announced via ProteomeXchange.

Title	TMT spectrometry unveils key M6a interactorsTMT spectrometry unveils key M6a interactors
Description	The membrane glycoprotein M6a -together with proteolipid protein (PLP), DM20 and M6b- belongs to the tetraspan PLP family. M6a is a neuronal surface protein that promotes neuronal stem cell differentiation, migration, neurite and axonal outgrowth, filopodia/spine induction and synapse formation in primary neuronal cultures and non-neuronal cell lines. M6a has two extracellular loops (EC1 and EC2), four transmembrane domains, one intracellular loop and the N and C terminus facing the cell cytoplasm. However, the complete mechanism of action or proteins associated with it through its extracellular loops remained unknown. In previous work we provided strong evidence that M6a´s extracellular loops widely contribute to its function. To asses this question, we designed and purified a chimera protein (M6a-loops as a bait protein) which was subjected to co-immunoprecipitation (Co-Ip) with rat hippocampi homogenates followed by TMT-MS. The TMT-MS and data analysis was done by the Proteomics Core Facility from the European Molecular Biology Laboratory (EMBL, Heidelberg, Germany).
HostingRepository	PRIDE
AnnounceDate	2020-06-29
AnnouncementXML	Submission_2020-09-01_04:42:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camila Scorticati
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-31 01:46:03	ID requested
1	2020-06-29 00:55:03	announced
2	2020-07-01 01:02:47	announced	2020-07-01: Updated publication reference for DOI(s): null.
⏵ 3	2020-09-01 04:42:52	announced	2020-07-01: Updated publication reference for DOI(s): null. 2020-09-01: Updated publication reference for PubMed record(s): 32848694. 2020-09-01: Updated publication reference for DOI(s): null.
4	2023-11-14 09:01:12	announced	2023-11-14: Updated project metadata.

Aparicio GI, Formoso K, Le, ó, n A, Frasch AC, Scorticati C, Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS. Front Synaptic Neurosci, 12():28(2020) [pubmed]

null;

submitter keyword: Glycoprotein M6a, Rattus norvegicus, Hippocampus, Co-immunoprecipitation, TMT-MS, protein-protein interactions

Camila Scorticati
contact affiliation	Group Leader. Laboratorio de Neurobiologia Molecular y Celular. Institutto de Investigaciones Biotecnologicas (IIBio) Consejo de Investigaciones Cientificas y Tecnicas (CONICET) Universidad Nacional de San Martin (UNSAM)
contact email	cscorticati@iib.unsam.edu.ar
lab head
Camila Scorticati
contact affiliation	Instituto de Investigaciones Biotecnológicas Dr. Rodolgo Ugalde - Universidad Nacional de San Martín
contact email	cscorticati@iibintech.com.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017347

PRIDE project URI

• PRIDE
  1. PXD017347
     1. Label: PRIDE project
     2. Name: TMT spectrometry unveils key M6a interactorsTMT spectrometry unveils key M6a interactors