PXD017330 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatio-temporal Proteomic Analysis of Stress Granule disassembly using APEX Reveals Regulation by SUMOylation and links to ALS pathogenesis |
Description | Cytoplasmic stress granules (SG) are membraneless organelles that form in response to a variety of cellular stresses by phase-separation of proteins associated with non-translating mRNAs. SG have recently been linked with neurodegeneration, including ALS, however there has been no systematic investigation of SG composition in normal and disease contexts. We created a proximity proteomics platform by using multiple ascorbate peroxidase (APEX2) baits to comprehensively characterize the spatio-temporal organization of SG in normal and disease (ALS-like) conditions. Multi-bait APEX enhanced sensitivity and enabled cross-validation, leading to the mass spectrometric discovery of 109 additional SG proteins, and internal SG substructures at proteomic resolution. A proteomic analysis of SG disassembly over time revealed a group of 349 proteins recruited specifically during SG disassembly, which we named disassembly-engaged proteins (DEPs), including SUMO ligases. A parallel study of SG disassembly in the presence of C9ORF72-associated dipeptides, which are found in patients with ALS and frontotemporal dementia revealed impaired SG disassembly and DEP recruitment, which may be linked to neurodegeneration. Finally, broad SUMOylation of SG proteins was required for SG disassembly, and impaired by C9ORF72-associated dipeptides. Altogether, our study provides a valuable resource, and dissects the SG spatio-temporal proteomic landscape, revealing basic and disease-relevant mechanisms of SG dynamics. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-19 |
AnnouncementXML | Submission_2020-10-18_23:13:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Geiger |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-30 01:17:44 | ID requested | |
⏵ 1 | 2020-10-18 23:13:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Stress granules, APEX, Proteomics, SUMOylation |
Contact List
Professor Tamar Geiger |
contact affiliation | Dept. of Human Molecular Genetics and Biochemistry Sackler Faculty of Medicine Tel Aviv University Israel |
contact email | geiger@tauex.tau.ac.il |
lab head | |
Tamar Geiger |
contact affiliation | Tel Aviv University |
contact email | geiger@tauex.tau.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017330
- Label: PRIDE project
- Name: Spatio-temporal Proteomic Analysis of Stress Granule disassembly using APEX Reveals Regulation by SUMOylation and links to ALS pathogenesis