PXD017300-1

PXD017300 is an original dataset announced via ProteomeXchange.

Title	LSD1 Prevents Aberrant Heterochromatin Formation in Neurospora crassa
Description	Heterochromatin is a specialized form of chromatin that restricts access to DNA and inhibits genetic processes, including transcription and recombination. In Neurospora crassa, constitutive heterochromatin is characterized by trimethylation of lysine 9 on histone H3, hypoacetylation of histones, and DNA methylation. Here we explore whether the conserved histone demethylase, lysine-specific demethylase 1 (LSD1), regulates heterochromatin in Neurospora, and if so, how. Though LSD1 is implicated in heterochromatin regulation, its function is inconsistent across different systems; orthologs of LSD1 have been shown to either promote or antagonize heterochromatin expansion by removing H3K4me or H3K9me respectively. We identify three members of the Neurospora LSD complex (LSDC): LSD1, PHF1, and BDP-1, and strains deficient for any exhibit variable spreading of heterochromatin and establishment of new heterochromatin domains dispersed across the genome. Heterochromatin establishment outside of canonical domains in Neurospora share the unusual characteristic of DNA methylation-dependent H3K9me3; typically, H3K9me3 establishment is independent of DNA methylation. Consistent with this, the hyper-H3K9me3 phenotype of LSD1 knock-out strains is dependent on the presence of DNA methylation, as well as HCHC-mediated histone deacetylation, suggesting spreading is dependent on some feedback mechanism. Altogether, our results suggest LSD1 works in opposition to HCHC to maintain proper heterochromatin boundaries.
HostingRepository	PRIDE
AnnounceDate	2021-07-21
AnnouncementXML	Submission_2021-07-21_15:07:18.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Neurospora crassa; NCBI TaxID: 5141;
ModificationList	monohydroxylated residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-28 02:39:09	ID requested
⏵ 1	2021-07-21 15:07:19	announced

Storck WK, Bicocca VT, Rountree MR, Honda S, Ormsby T, Selker EU, LSD1 prevents aberrant heterochromatin formation in Neurospora crassa. Nucleic Acids Res, 48(18):10199-10210(2020) [pubmed]

submitter keyword: Neurospora crassa, heterochromatin, lysine-specific demethylase, immunoprecipitation mass spectrometry

Eric U. Selker
contact affiliation	Dr. Eric U. Selker Institute of Molecular Biology University of Oregon Eugene, Oregon 97403, USA
contact email	selker@uoregon.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017300
     1. Label: PRIDE project
     2. Name: LSD1 Prevents Aberrant Heterochromatin Formation in Neurospora crassa